Forskarprestationer inom dedicerade Formasområden och

L_2008146SV.01001201.xml - EUR-Lex

Organism. Anaerobranca gottschalkii. Status. Unreviewed-Annotation score: -Protein inferred from homology i. Function i Cofactor i. Ca 2+ ARBA annotation.

Cgtase

24B Korpraditskul,. Prof. Dr. Vichai. Agrarwissen- schaft. Enzymeteknikmetoden är användningen av cyklodextrinkomplexglukosyltransferas (CGTase) eller pyruviskt enzym och andra monosackarider [14] Commonly cyclodextrin glycosyltransferase (CGTase) is employed along with α-amylase.

Screening och val av medelkomponenter för cyklodextrin

alpha-amylase activity Source: InterPro 2016-05-07 cgtase bacteria cyclodextrin gene starch Prior art date 1990-03-27 Application number PCT/EP1991/000560 Other languages German (de) French (fr) Other versions WO1991014770A3 (en Inventor Gerhard Schmid Original Assignee Consortium für elektrochemische Industrie GmbH Priority date (The priority date is an assumption and is not a legal conclusion. 2012-12-12 2016-04-15 CGTase was expressed by recombinant K. phaffii through pH maintenance in range of 5.5–7.0. β‐CGTase activity increased to 122.0 U/mL after optimization of glycerol, phosphate buffer, pH value, ammonium sulfate, temperature, methanol, and additives based on BSM, establishing a modified defined medium. For CGTase this mechanism can easily be imagined with the aid of a maltononaose substrate bound in the active site , as derived from the X‐ray structure of the enzyme complexed with a maltononaose inhibitor [].

Kumaraswamy · Production of CGTase from B Book 2020 - Svenska

Das Produkt wird hergestellt durch Einwirkung des Enzyms Cycloglykosyltransferase (CGTase), gewonnen aus Bacillus circulans, Paenibacillus macerans bzw. The CGTase gene from the hyperthermophilic archaea Archaeoglobus fulgidus (AfCGTase) has 34 % rare codons as compared to Escherichia coli genes. Het product wordt verkregen door de inwerking van het enzym cycloglycosyltransferase (CGTase) uit Bacillus circulans, Paenibacillus macerans of recombinant Glycosyltransferas (CGTase).

alpha-amylase activity Source: InterPro 2021-02-20 2002-09-12 Cyclodextrin glucanotransferases (CGTase; E.C 2.4.1.19) belonging to the glycoside hydrolase family 13 (GH 13) are widely used as catalysts in starch conversion processes (Han et al. 2014). CGTases mainly perform three different reactions in addition to hydrolysis, namely … The product specificity and pH optimum of the thermostable cyclodextrin glycosyltransferase (CGTase) from Thermoanaerobacterium thermosulfurigenes EM1 was engineered using a combination of x-ray crystallography and site-directed mutagenesis.
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The enzyme was purified and displayed optimum activity at 50°C and pH 6 … One family member, cyclodextrin glycosyltransferase (CGTase), has an uncommonly high transglycosylation activity and is able to form cyclodextrins.

cgtase - Cyclodextrin glycosyltransferase - Anaerobranca gottschalkii - cgtase gene & protein UniProtKB - Q5ZEQ7 (Q5ZEQ7_9FIRM) γ-Cyclodextrin glycosyltransferase (γ-CGTase) catalyzes the biotransformation of low-cost starch into valuable γ-cyclodextrin (γ-CD), which is widely applied in biotechnology, food, and pharmaceutical industries. CGTase is an industrially important enzyme for α-, β- or γ-cyclodextrins (CDs) production, which are extensively used in agriculture, chemicals, cosmetics, foods and pharmaceuticals .
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Purified CGTase showed 5.18 kcal/mol activation energy (Ea). Objectives: Cyclodextrin glucanotransferase (CGTase) is a multifunctional industrial enzyme, which undergoes cyclization reaction to converts starch into Cyclodextrin (CD). Due to their potential properties, CDs had been discovered to more. The cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) gene from Bacillus sp. G1 was successfully isolated and cloned into Escherichia coli. Analysis of the nucleotide sequence revealed the presence of an open reading frame of 2,109 bp and encoded The CGTase enzyme obtained from the M5 clone had Km and Vmax values 13.59 mg/mL and 1153 µmol/(mg min), respectively.

Forskarprestationer inom dedicerade Formasområden och

Den framställs genom att glukosyltransferas (CGTase, EC 2.4.1.19) får reagera med hydrolyserad stärkelse. Återvinning och rening av α-cyklodextrin kan ske A FI 892927A FI 98737 C FI98737 C FI 98737C; Authority: FI: Finland; Prior art keywords: starch: cgtase: cyclodextrin: strain: ncib; Prior art date: 1987-10-15. 2 344 4 a- Bacillus megaterium B. macerans CGTase 22 C-2, C-3, C-4 5) CGTase 6) , ) 10 8) 1, 3 Suzuki B. stearothermophilus CGTase Rhizopus sp. Phase 2: CGTase-catalyzed elongation of carboxylated alkyl glycosides - Design a set of experiments - Analyze data from HPLC, UV, LC-MS, NMR - Scale-up 219, nr 2, s. 181-185.

Due to their potential properties, CDs had been discovered to more. The cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) gene from Bacillus sp. G1 was successfully isolated and cloned into Escherichia coli. Analysis of the nucleotide sequence revealed the presence of an open reading frame of 2,109 bp and encoded The CGTase enzyme obtained from the M5 clone had Km and Vmax values 13.59 mg/mL and 1153 µmol/(mg min), respectively. The enzyme was purified and displayed optimum activity at 50°C and pH 6 … One family member, cyclodextrin glycosyltransferase (CGTase), has an uncommonly high transglycosylation activity and is able to form cyclodextrins.